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KMID : 0613820080180020287
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Journal of Life Science
2008 Volume.18 No. 2 p.287 ~ p.290
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Establishment of an Antibacterial Yeast That Producing Bacteriocin Subpeptin JM4-A or Subpeptin JM4-B
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Lee Ok-Hee
Jang Min-Kyung
Lee Dong-Geun
Kim In-Hae
Lee Jae-Hwa
Ha Jong-Myuong
Ha Bae-Jin
Ahn Ik-Yong
Cho Dong-In
Lee Sang-Hyun
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Abstract
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In order to obtain yeast cells producing a bacteriocin, Subpeptin JM4-A or Subpeptin JM4-B, the 48 bp oligonucleotides corresponding to Subpeptin JM4-A and Subpeptin JM4-B genes including codon for start and stop were chemically synthesized and cloned into pAUR123, an yeast expression vector. Transformed yeast cells exhibited growth inhibition of Bacillus subtilis, Escherichia coli and Pseudonomas aeruginosa. This result indicates that yeast cells producing Subpeptin JM4-A or Subpeptin JM4-B possess bacteriocidal properties against both Gram positive B. subtilis and Gram negative E. coli and P. aeruginosa cells. The recombinant yeast strains constructed in this study can be applied in the food preservative or. animal foodfeed.
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KEYWORD
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Bacteriocin, Pseudomonas aeruginosa, Saccharomyces cerevisiae, Subpeptin JM4-A, Subpeptin JM4-B
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